Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Tissue factor detection in mouse tissues by western blotting using commercial antibodies

doi: 10.1016/j.rpth.2025.103336

Figure Lengend Snippet: Detection of tissue factor (TF) in a mouse cell line using 4 different commercial anti-mouse TF antibodies. Proteins in cell lysates (10 μg) from the mouse pancreatic cancer cell lines KPC2 wild-type (WT) and KPC2 TF knockout (KO) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to a membrane. TF was detected using 4 commercial anti-mouse TF antibodies: (A) Santa Cruz sc-374441 mouse anti-mouse TF monoclonal antibody, (B) R&D Systems (R&D) AF3178 goat anti-mouse TF polyclonal antibody, (C) Abcam ab189483 rabbit anti-mouse TF monoclonal antibody, or (D) Cell Signaling Technology (Cell Signaling) 44861 rabbit anti-mouse TF monoclonal antibody. These anti-TF antibodies were detected using species-specific secondary antibodies . β-Actin was used as a loading control and detected with an anti–β-actin antibody . The binding of the anti–β-actin antibody was detected with a horse anti-mouse immunoglobulin G horseradish peroxidase-linked antibody . For imaging, the blots were exposed for 1 second for both TF and β-actin. All blots were visualized using the iBright FL1000 imaging system. kDa, kilodalton.

Article Snippet: We evaluated the ability of the following commercially available antibodies to detect TF in KPC2 WT cells: goat polyclonal anti-mouse TF antibody AF3178 (R&D Systems), rabbit monoclonal anti-mouse TF antibody ab189483 (Abcam), rabbit monoclonal anti-mouse TF antibody 44861 (Cell Signaling Technology), and mouse monoclonal anti-mouse TF antibody H-9 (Santa Cruz; ).

Techniques: Knock-Out, Polyacrylamide Gel Electrophoresis, Membrane, Control, Binding Assay, Imaging

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Tissue factor detection in mouse tissues by western blotting using commercial antibodies

doi: 10.1016/j.rpth.2025.103336

Figure Lengend Snippet: Detection of tissue factor (TF) in mouse tissues using the R&D Systems antibody. (A) Proteins in lysates or (B) control or deglycosylated lysates from the indicated wild-type (WT) or low TF (LTF) mouse organs (40 μg) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to membranes. TF was detected using the R&D Systems AF3178 goat anti-mouse TF polyclonal antibody. The binding of the R&D Systems anti-TF antibody was detected with a rabbit anti-goat immunoglobulin G horseradish peroxidase-linked antibody . α-Actinin was used as a loading control and detected with an anti–α-actinin antibody . The binding of the anti–α-actinin antibody was detected with a goat anti-rabbit immunoglobulin G horseradish peroxidase-linked antibody . For imaging, the TF blots were exposed for 1 second. For α-actinin, the blots were exposed for (A) 15 or (B) 30 seconds. All blots were visualized using the iBright FL1000 imaging system. ab, antibody; dgTF, deglycosylated tissue factor; gTF, glycosylated tissue factor; kDa, kilodalton.

Article Snippet: We evaluated the ability of the following commercially available antibodies to detect TF in KPC2 WT cells: goat polyclonal anti-mouse TF antibody AF3178 (R&D Systems), rabbit monoclonal anti-mouse TF antibody ab189483 (Abcam), rabbit monoclonal anti-mouse TF antibody 44861 (Cell Signaling Technology), and mouse monoclonal anti-mouse TF antibody H-9 (Santa Cruz; ).

Techniques: Control, Polyacrylamide Gel Electrophoresis, Binding Assay, Imaging

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Tissue factor detection in mouse tissues by western blotting using commercial antibodies

doi: 10.1016/j.rpth.2025.103336

Figure Lengend Snippet: Detection of tissue factor (TF) in a mouse cell line using 4 different commercial anti-mouse TF antibodies. Proteins in cell lysates (10 μg) from the mouse pancreatic cancer cell lines KPC2 wild-type (WT) and KPC2 TF knockout (KO) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to a membrane. TF was detected using 4 commercial anti-mouse TF antibodies: (A) Santa Cruz sc-374441 mouse anti-mouse TF monoclonal antibody, (B) R&D Systems (R&D) AF3178 goat anti-mouse TF polyclonal antibody, (C) Abcam ab189483 rabbit anti-mouse TF monoclonal antibody, or (D) Cell Signaling Technology (Cell Signaling) 44861 rabbit anti-mouse TF monoclonal antibody. These anti-TF antibodies were detected using species-specific secondary antibodies . β-Actin was used as a loading control and detected with an anti–β-actin antibody . The binding of the anti–β-actin antibody was detected with a horse anti-mouse immunoglobulin G horseradish peroxidase-linked antibody . For imaging, the blots were exposed for 1 second for both TF and β-actin. All blots were visualized using the iBright FL1000 imaging system. kDa, kilodalton.

Article Snippet: TF was detected using the R&D Systems AF3178 goat anti-mouse TF polyclonal antibody.

Techniques: Knock-Out, Polyacrylamide Gel Electrophoresis, Membrane, Control, Binding Assay, Imaging

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Tissue factor detection in mouse tissues by western blotting using commercial antibodies

doi: 10.1016/j.rpth.2025.103336

Figure Lengend Snippet: Detection of tissue factor (TF) in mouse tissues using the R&D Systems antibody. (A) Proteins in lysates or (B) control or deglycosylated lysates from the indicated wild-type (WT) or low TF (LTF) mouse organs (40 μg) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to membranes. TF was detected using the R&D Systems AF3178 goat anti-mouse TF polyclonal antibody. The binding of the R&D Systems anti-TF antibody was detected with a rabbit anti-goat immunoglobulin G horseradish peroxidase-linked antibody . α-Actinin was used as a loading control and detected with an anti–α-actinin antibody . The binding of the anti–α-actinin antibody was detected with a goat anti-rabbit immunoglobulin G horseradish peroxidase-linked antibody . For imaging, the TF blots were exposed for 1 second. For α-actinin, the blots were exposed for (A) 15 or (B) 30 seconds. All blots were visualized using the iBright FL1000 imaging system. ab, antibody; dgTF, deglycosylated tissue factor; gTF, glycosylated tissue factor; kDa, kilodalton.

Article Snippet: TF was detected using the R&D Systems AF3178 goat anti-mouse TF polyclonal antibody.

Techniques: Control, Polyacrylamide Gel Electrophoresis, Binding Assay, Imaging

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Tissue factor detection in mouse tissues by western blotting using commercial antibodies

doi: 10.1016/j.rpth.2025.103336

Figure Lengend Snippet: Detection of tissue factor (TF) in a mouse cell line using 4 different commercial anti-mouse TF antibodies. Proteins in cell lysates (10 μg) from the mouse pancreatic cancer cell lines KPC2 wild-type (WT) and KPC2 TF knockout (KO) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to a membrane. TF was detected using 4 commercial anti-mouse TF antibodies: (A) Santa Cruz sc-374441 mouse anti-mouse TF monoclonal antibody, (B) R&D Systems (R&D) AF3178 goat anti-mouse TF polyclonal antibody, (C) Abcam ab189483 rabbit anti-mouse TF monoclonal antibody, or (D) Cell Signaling Technology (Cell Signaling) 44861 rabbit anti-mouse TF monoclonal antibody. These anti-TF antibodies were detected using species-specific secondary antibodies . β-Actin was used as a loading control and detected with an anti–β-actin antibody . The binding of the anti–β-actin antibody was detected with a horse anti-mouse immunoglobulin G horseradish peroxidase-linked antibody . For imaging, the blots were exposed for 1 second for both TF and β-actin. All blots were visualized using the iBright FL1000 imaging system. kDa, kilodalton.

Article Snippet: We used the goat anti-mouse TF polyclonal antibody AF3178 (R&D Systems), the rabbit anti-mouse TF monoclonal antibody ab189483 (Abcam), the rabbit anti-mouse TF monoclonal antibody 44861 (Cell Signaling Technology), and the mouse anti-mouse TF monoclonal antibody H9 (Santa Cruz Biotechnology) to measure TF in the mouse pancreatic cancer cell line KPC2 wild-type and KPC2 TF knockout cell lysates, as well as various tissues from wild-type and low TF mice (no mouse TF expression) using western blotting.

Techniques: Knock-Out, Polyacrylamide Gel Electrophoresis, Membrane, Control, Binding Assay, Imaging

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Tissue factor detection in mouse tissues by western blotting using commercial antibodies

doi: 10.1016/j.rpth.2025.103336

Figure Lengend Snippet: Detection of tissue factor (TF) in mouse tissues using the R&D Systems antibody. (A) Proteins in lysates or (B) control or deglycosylated lysates from the indicated wild-type (WT) or low TF (LTF) mouse organs (40 μg) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to membranes. TF was detected using the R&D Systems AF3178 goat anti-mouse TF polyclonal antibody. The binding of the R&D Systems anti-TF antibody was detected with a rabbit anti-goat immunoglobulin G horseradish peroxidase-linked antibody . α-Actinin was used as a loading control and detected with an anti–α-actinin antibody . The binding of the anti–α-actinin antibody was detected with a goat anti-rabbit immunoglobulin G horseradish peroxidase-linked antibody . For imaging, the TF blots were exposed for 1 second. For α-actinin, the blots were exposed for (A) 15 or (B) 30 seconds. All blots were visualized using the iBright FL1000 imaging system. ab, antibody; dgTF, deglycosylated tissue factor; gTF, glycosylated tissue factor; kDa, kilodalton.

Article Snippet: We used the goat anti-mouse TF polyclonal antibody AF3178 (R&D Systems), the rabbit anti-mouse TF monoclonal antibody ab189483 (Abcam), the rabbit anti-mouse TF monoclonal antibody 44861 (Cell Signaling Technology), and the mouse anti-mouse TF monoclonal antibody H9 (Santa Cruz Biotechnology) to measure TF in the mouse pancreatic cancer cell line KPC2 wild-type and KPC2 TF knockout cell lysates, as well as various tissues from wild-type and low TF mice (no mouse TF expression) using western blotting.

Techniques: Control, Polyacrylamide Gel Electrophoresis, Binding Assay, Imaging